Reconstitution is the process of dissolving lyophilized peptide powder in a solvent to create a solution suitable for research use. Proper technique ensures sterility, accuracy, and peptide stability.
Materials Needed
- Bacteriostatic water (0.9% benzyl alcohol) or sterile saline
- Sterile syringes and needles
- Alcohol swabs
- Calculator for dose/concentration
Step-by-Step Process
- Allow the peptide vial to reach room temperature.
- Wipe the vial stopper and solvent vial with alcohol.
- Draw the desired volume of solvent into a syringe.
- Inject the solvent slowly against the vial wall to avoid foaming.
- Gently swirl or roll the vial—do not shake vigorously.
- Allow the solution to fully dissolve (may take a few minutes).
- Label the vial with concentration, date, and contents.
Solvent Selection
Bacteriostatic water is standard for most peptides. Some peptides may require acetic acid or other solvents—check product documentation. Use only sterile, research-grade solvents.